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v fluvialis atcc 33809  (ATCC)


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    ATCC v fluvialis atcc 33809
    Characterization of the homologous sequences of the gene cluster for vibriobactin biosynthesis in pathogenic Vibrios . (A) Illustration of the homologous sequences of the gene cluster for vibriobactin biosynthesis and the adjacent genes for vibriobactin transport in pathogenic Vibrios . For the representative strain of each species (the strain names were shown next to the species names), the NCBI accession numbers and the locus_tags of homologous genes were shown. The known homologous genes for vibriobactin biosynthesis ( vibABCDEFH ) and their adjacent genes for vibriobactin transport ( viuPDGC ) in V. cholerae O395 were shown in different colors, while the unmatched genes in the strains of other species were shown in grey. The length scale represented 2.5 kb. (B) Illustration of the genes for fluvibactin biosynthesis ( vfbABCDEFH ), their adjacent genes for fluvibactin transport ( vfuPDGC ) and other genes with unknown functions (the locus_tags of genes in V. fluvialis 33809 were shown) in V. fluvialis 85003. For genes used to test for transcript units, the lengths of intergenic regions were indicated. The A I, A II, A III, and A IV indicated the primer pairs used to test for transcript units in panel (C) . The I–IX indicated the nine transcription units of the gene cluster for fluvibactin biosynthesis, as confirmed in panel (C) . (C) RT-PCR analysis of the transcriptional units of the gene cluster for fluvibactin biosynthesis. The cDNA indicated the complementary DNA reverse-transcribed from RNA. The gDNA (genomic DNA) and RNA (RNA without reverse transcription) were served as positive and negative controls, respectively. The M indicated marker.
    V Fluvialis Atcc 33809, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Quorum sensing regulators AphA and HapR differentially regulate fluvibactin biosynthesis in Vibrio fluvialis"

    Article Title: Quorum sensing regulators AphA and HapR differentially regulate fluvibactin biosynthesis in Vibrio fluvialis

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2026.1829491

    Characterization of the homologous sequences of the gene cluster for vibriobactin biosynthesis in pathogenic Vibrios . (A) Illustration of the homologous sequences of the gene cluster for vibriobactin biosynthesis and the adjacent genes for vibriobactin transport in pathogenic Vibrios . For the representative strain of each species (the strain names were shown next to the species names), the NCBI accession numbers and the locus_tags of homologous genes were shown. The known homologous genes for vibriobactin biosynthesis ( vibABCDEFH ) and their adjacent genes for vibriobactin transport ( viuPDGC ) in V. cholerae O395 were shown in different colors, while the unmatched genes in the strains of other species were shown in grey. The length scale represented 2.5 kb. (B) Illustration of the genes for fluvibactin biosynthesis ( vfbABCDEFH ), their adjacent genes for fluvibactin transport ( vfuPDGC ) and other genes with unknown functions (the locus_tags of genes in V. fluvialis 33809 were shown) in V. fluvialis 85003. For genes used to test for transcript units, the lengths of intergenic regions were indicated. The A I, A II, A III, and A IV indicated the primer pairs used to test for transcript units in panel (C) . The I–IX indicated the nine transcription units of the gene cluster for fluvibactin biosynthesis, as confirmed in panel (C) . (C) RT-PCR analysis of the transcriptional units of the gene cluster for fluvibactin biosynthesis. The cDNA indicated the complementary DNA reverse-transcribed from RNA. The gDNA (genomic DNA) and RNA (RNA without reverse transcription) were served as positive and negative controls, respectively. The M indicated marker.
    Figure Legend Snippet: Characterization of the homologous sequences of the gene cluster for vibriobactin biosynthesis in pathogenic Vibrios . (A) Illustration of the homologous sequences of the gene cluster for vibriobactin biosynthesis and the adjacent genes for vibriobactin transport in pathogenic Vibrios . For the representative strain of each species (the strain names were shown next to the species names), the NCBI accession numbers and the locus_tags of homologous genes were shown. The known homologous genes for vibriobactin biosynthesis ( vibABCDEFH ) and their adjacent genes for vibriobactin transport ( viuPDGC ) in V. cholerae O395 were shown in different colors, while the unmatched genes in the strains of other species were shown in grey. The length scale represented 2.5 kb. (B) Illustration of the genes for fluvibactin biosynthesis ( vfbABCDEFH ), their adjacent genes for fluvibactin transport ( vfuPDGC ) and other genes with unknown functions (the locus_tags of genes in V. fluvialis 33809 were shown) in V. fluvialis 85003. For genes used to test for transcript units, the lengths of intergenic regions were indicated. The A I, A II, A III, and A IV indicated the primer pairs used to test for transcript units in panel (C) . The I–IX indicated the nine transcription units of the gene cluster for fluvibactin biosynthesis, as confirmed in panel (C) . (C) RT-PCR analysis of the transcriptional units of the gene cluster for fluvibactin biosynthesis. The cDNA indicated the complementary DNA reverse-transcribed from RNA. The gDNA (genomic DNA) and RNA (RNA without reverse transcription) were served as positive and negative controls, respectively. The M indicated marker.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Marker

    Related Articles

    In Vitro:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Control:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Positive Control:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Polymerase Chain Reaction:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Membrane:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Amplification:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Sterility:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Saline:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr

    Incubation:

    Article Title: A Fitness Burden Drives the Reversion of in vitro Acquired Doxycycline Resistance in V. cholerae O1
    Article Snippet: e selective medium Vibrio chromagar™ (CHROMagar, Paris, France, VB912), and single colonies were selected for downstream assays to ensure culture purity and consistency. A reference V. cholerae strain ATCC 14035 (strain 8021) was included as a quality control in antimicrobial susceptibility testing to ensure assay accuracy and reproducibility. Culture purity was verified by colony morphology and gr



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    Characterization of the homologous sequences of the gene cluster for vibriobactin biosynthesis in pathogenic Vibrios . (A) Illustration of the homologous sequences of the gene cluster for vibriobactin biosynthesis and the adjacent genes for vibriobactin transport in pathogenic Vibrios . For the representative strain of each species (the strain names were shown next to the species names), the NCBI accession numbers and the locus_tags of homologous genes were shown. The known homologous genes for vibriobactin biosynthesis ( vibABCDEFH ) and their adjacent genes for vibriobactin transport ( viuPDGC ) in V. cholerae O395 were shown in different colors, while the unmatched genes in the strains of other species were shown in grey. The length scale represented 2.5 kb. (B) Illustration of the genes for fluvibactin biosynthesis ( vfbABCDEFH ), their adjacent genes for fluvibactin transport ( vfuPDGC ) and other genes with unknown functions (the locus_tags of genes in V. fluvialis 33809 were shown) in V. fluvialis 85003. For genes used to test for transcript units, the lengths of intergenic regions were indicated. The A I, A II, A III, and A IV indicated the primer pairs used to test for transcript units in panel (C) . The I–IX indicated the nine transcription units of the gene cluster for fluvibactin biosynthesis, as confirmed in panel (C) . (C) RT-PCR analysis of the transcriptional units of the gene cluster for fluvibactin biosynthesis. The cDNA indicated the complementary DNA reverse-transcribed from RNA. The gDNA (genomic DNA) and RNA (RNA without reverse transcription) were served as positive and negative controls, respectively. The M indicated marker.

    Journal: Frontiers in Microbiology

    Article Title: Quorum sensing regulators AphA and HapR differentially regulate fluvibactin biosynthesis in Vibrio fluvialis

    doi: 10.3389/fmicb.2026.1829491

    Figure Lengend Snippet: Characterization of the homologous sequences of the gene cluster for vibriobactin biosynthesis in pathogenic Vibrios . (A) Illustration of the homologous sequences of the gene cluster for vibriobactin biosynthesis and the adjacent genes for vibriobactin transport in pathogenic Vibrios . For the representative strain of each species (the strain names were shown next to the species names), the NCBI accession numbers and the locus_tags of homologous genes were shown. The known homologous genes for vibriobactin biosynthesis ( vibABCDEFH ) and their adjacent genes for vibriobactin transport ( viuPDGC ) in V. cholerae O395 were shown in different colors, while the unmatched genes in the strains of other species were shown in grey. The length scale represented 2.5 kb. (B) Illustration of the genes for fluvibactin biosynthesis ( vfbABCDEFH ), their adjacent genes for fluvibactin transport ( vfuPDGC ) and other genes with unknown functions (the locus_tags of genes in V. fluvialis 33809 were shown) in V. fluvialis 85003. For genes used to test for transcript units, the lengths of intergenic regions were indicated. The A I, A II, A III, and A IV indicated the primer pairs used to test for transcript units in panel (C) . The I–IX indicated the nine transcription units of the gene cluster for fluvibactin biosynthesis, as confirmed in panel (C) . (C) RT-PCR analysis of the transcriptional units of the gene cluster for fluvibactin biosynthesis. The cDNA indicated the complementary DNA reverse-transcribed from RNA. The gDNA (genomic DNA) and RNA (RNA without reverse transcription) were served as positive and negative controls, respectively. The M indicated marker.

    Article Snippet: Homologous gene clusters were identified in eight pathogenic Vibrio species, with the names of representative strains and the corresponding accession numbers as follow: V. harveyi SB1 ( CP125875.1 ), V. vulnificus NBRC 15645 ( CP012882.1 ), V. fluvialis ATCC 33809 ( CP014035.2 ), V. furnissii FDAARGOS_777 ( CP040990.1 ), V. anguillarum PF4-E1-1 ( CP031479.1 ) and V. splendidus 2_C04b ( CP089203.1 ), V. metschnikovii NCTC 8443 ( UHIH01000002.1 ), V. campbellii BoB-53 ( CP026321.1 ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Marker